The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: This work was supported by Guangdong Medical Research Foundation (B2013220), and Youth Science and technology personnel training project of “Scientific research plan” of Southern Medical University, and an “Outstanding Leadership Grant” from Guangdong Province (C1030925), and Key Project of Science and Technology Research grants from the Ministry of Education (B1000409). Received: MaAccepted: Published: July 19, 2013Ĭopyright: © 2013 Liu et al. PLoS ONE 8(7):Įditor: Rajvir Dahiya, UCSF/VA Medical Center, United States of America MiR-196b may represent an effective target for chronic myeloid leukemia therapy.Ĭitation: Liu Y, Zheng W, Song Y, Ma W, Yin H (2013) Low Expression of miR-196b Enhances the Expression of BCR-ABL1 and HOXA9 Oncogenes in Chronic Myeloid Leukemogenesis. A low level of expression of miR-196b can cause up-regulation of BCR-ABL1 and HOXA9 expression, which leads to the development of chronic myeloid leukemia. Further examination of cell function indicated that miR-196b acts to reduce BCR-ABL1 and HOXA9 protein levels, decrease cell proliferation rate and retard the cell cycle. The dual-luciferase reporter assay system demonstrated that BCR-ABL1 and HOXA9 are the target genes of miR-196b, which was consistent with predictions from bioinformatics software analyses. The CpG islands showed more methylation in patients with chronic myeloid leukemia compared with healthy individuals (P<0.05), which indicated that low expression of miR-196b may be associated with an increase in the methylation of CpG islands. Bisulfite genomic sequencing PCR and methylation-specific PCR were used to examine the methylation status of the CpG islands in the miR-196b promoter in K562 cells, patients with leukemia and healthy individuals. In this study, low levels of expression of miR-196b were detected in patients with chronic myeloid leukemia. MicroRNAs (miRNAs) can function as tumor suppressors or oncogene promoters during tumor development.
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